In the present work, air-dried leaves from Khaya senegalensis seedlings that was irrigated with different irrigation schedules using agricultural drainage water and fertilized with seaweed extract, certain biological fertilizers, and mineral fertilizers—as well as their combinations on the quantity of crude extracts. The crude extracts were carried out using the ethanol solvent and expressed as g/100 g of air-dried leaves. Regarding the irrigation regime's influence, the irrigation that uses 50% Agricultural Drainage Water (ADW) and 50% Nile Water (NW) has produced the highest amount of leaf extract (8.558 g/100 g of air-dried leaves). The seedlings that were fertilized with Biofertilizer (30 ml/bag) containing strains (TS)® (Bio-enriched fluid, Bio Tech Company) comprising Bacillus circulans, Bacillus polymyxa, Bacillus megaterium, Candida spp., and Trichoderma spp. showed the highest amount of leaf extract, measuring 9.123 g/100 g of air-dried leaves. This indicates the impact of fertilization. The seedlings irrigated with 50% NW + 50% ADW and fertilized with TS 30 ml/bag showed the highest LE (10.567 g/100 g of air-dried leaves) about the significant interaction between the effects of the irrigation regime and fertilization. These seedlings were followed by those irrigated with 100% ADW + without fertilizer (10.372 g/100 g of air-dried leaves).