The natural extracts of studied plants may provide a new source of antimicrobial agents. The aim of this thesis is to isolated and assess the effectiveness and efficiency of five plant extracts from different parts as an antibacterial and antifungal. Extract content (EC%) of tested plants of leaf and branch of Callistemon viminalis was (59.49 and 4.37 EC %, respectively), Phytolacca dioica (3.14 and 1.35 EC %, respectively) and Sophora secundiflora (5.21 and 4.90 EC %, respectively), as well as the leaf, branch and seed of each Millettia pinnata (3.18, 3.33 and 2.28 EC %, respectively) and Lagunaria patersonii (2.12, 2.82 and 2.47 EC %, respectively) that had been tested on the fungi (Alternaria solani, Bipolaris oryzae, Fusarium oxysporum and Rhizoctonia solani). Also on the bacteria (Agrobacterium tumefaciens, Erwinia amylovora, Pectobacterium carotovorum subsp. Carotovorum and Ralstonia solanacerum).