The experiments of the present study were conducted in the Plant Tissue Culture and Biotechnology Labs, Maryuot Research station, Desert Research Center, Egypt and the Department of genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. Seriphidium herba-album is very important medicinal plants that have been used in folk medicine for many years till now for the treatment of gastric disturbances and healing external wounds, remission of diabetic symptoms, activating of the liver and healing rashes, joint pains, inflammations and rheumatoid arthritis, with no side-effects. S. herba-album belongs toArtemisia genus, family of Asteraceae which contains about 23000 species. Artemisia genus plant produces a various number of secondary metabolites that showed biological activity. Artemisinin is sesquiterpene lactones. This substance is known for its medical efficacy, antioxidant, strong anti-inflammatory, antitumor activity, antimalarial, and for the fact Artemisinin could increase immunity.