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357593

Laboratory diagnosis and treatment of pathogenic bacteria isolated from children with cervical lymphadenopathy at Tanta University Hospital

Article

Last updated: 23 Dec 2024

Subjects

-

Tags

Human Microbial Interactions
Medical Microbiology

Abstract

Immune illness, cancer, and microbial infections can cause lymphadenopathy, which enlarges lymph nodes. Current research focuses on laboratory diagnosis of microbial infections that cause lymphadenopathy in children by isolating and identifying microorganisms from throat and lymph node aspiration, performing throat and lymph node aspiration cultures, and treating the most common isolated bacterial infections with bioproducts. Children shown lymphadenopathy were microbiologically examined. Bacterial taxa recovered from cervical lymphadenopathy patients were Staphylococcus aureus (MRSA) and Streptococcus pyogenes. The two isolates were identified molecularly, and their sequences had been deposited in GenBank under the accession numbers PP527743 and PP527744 respectively. The agar well diffusion test assessed the antimicrobial properties of the clove, garlic, thyme oils and Aloe vera gel. Comparatively, garlic oil was the best antibacterial bioproduct against isolated bacteria, with inhibition zones of 20- and 21-mm. respectively. The minimum garlic inhibitory concentrations against MRSA and S. pyogenes were 6.25% and 0.19%. Cefotaxime antibiotic came first in comparison with other used antibiotics (Vancomycin, Ampicillin/sulbactam, Penicillin G) against common isolated bacteria and the mean diameter of inhibition zones  were 31 mm and 35 mm, respectively. This study concluded that, the most common isolated bacterial infections from children with cervical lymphadenopathy were methicillin-resistant S. aureus and S. pyogenes. Among the several bioproducts tested against bacteria recovered from children with cervical lymphadenopathy, garlic oil demonstrated the highest antibacterial efficacy.

DOI

10.21608/mb.2024.283357.1095

Keywords

Bioproducts, Egypt, garlic oil, microbial infections, Staphylococcus aureus (MRSA), Streptococcus pyogenes

Authors

First Name

Saida

Last Name

Amer

MiddleName

M.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Tanta University, Egypt.

Email

saida_amer2014@yahoo.com

City

-

Orcid

-

First Name

Nanis

Last Name

Allam

MiddleName

G.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Tanta University, Egypt.

Email

ngamal1973@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

El-shanshory

MiddleName

R.

Affiliation

Department of Pediatrics, Faculty of Medicine, Tanta University, Egypt.

Email

mohamedmoustafafdd100@gmail.com

City

-

Orcid

-

First Name

Fatma

Last Name

Ibrahim

MiddleName

A.

Affiliation

Department of Biochemistry, National Research Center, Egypt.

Email

fatmaibrahim2020@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

El-shafey

MiddleName

S.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Tanta University, Egypt.

Email

ahmedsamymmd@gmail.com

City

-

Orcid

-

Volume

9

Article Issue

1

Related Issue

47475

Issue Date

2024-06-01

Receive Date

2024-04-17

Publish Date

2024-06-01

Page Start

63

Page End

75

Print ISSN

2357-0326

Online ISSN

2357-0334

Link

https://mb.journals.ekb.eg/article_357593.html

Detail API

https://mb.journals.ekb.eg/service?article_code=357593

Order

357,593

Type

Original Article

Type Code

502

Publication Type

Journal

Publication Title

Microbial Biosystems

Publication Link

https://mb.journals.ekb.eg/

MainTitle

Laboratory diagnosis and treatment of pathogenic bacteria isolated from children with cervical lymphadenopathy at Tanta University Hospital

Details

Type

Article

Created At

23 Dec 2024