Type 2 Diabetes and its complications is a worldwide epidemic and a significant cause of illness and death. The specific relation between vitamin D level and immunity status in Diabetes is still poorly understood. This research may help create new potential therapies to prevent diabetic complications. To find out the impact of vitamin D dietary supplementation on Type 2 diabetes immunological aspects, an experimental randomized prospective study was carried out in the Medical Physiology Department, Medical division, National Research Center on thirty albino male rats. Three groups—negative control, positive diabetic control, and diabetic group with intramuscular injections of 20,000 IU/kg Vitamin D3 were included in the study. Insulin, fasting blood glucose (FBG), homeostasis model assessment for insulin resistance (HOMA-IR) and inflammatory markers were measured in all groups. HOMA-IR, nuclear factor kappa B (NF-KB), tumor necrosis factor-α (TNF-α), Interleukin 1B (IL-1B), C reactive protein (CRP) and immunoglobulin A (IgA), were considerably lower in treated group than positive control group. After treatment, glucose was significantly lowered in the group treated with vitamin D3. As a result, vitamin D3 i.m administration may enhance glycemic management in those animals with type 2 diabetes mellitus.