This research deals with using agricultural waste (rice straw) to make fertilizers through bokashi or as compost in a laboratory scale. The first experiment was preparation of anaerobic bokashi is made in closed vessels by using an organic material which was inoculated with the microbial inoculants. Laboratory experiment was conducted in six treatments and incubated for 15 days then dried and analyzed to select the best treatment for a pot experiment. It was carried out in Agricultural Research Center, Giza, Egypt, to study the influence of rice straw as three types incorporating (rice straw, bokashi and compost) with three level of mineral fertilizer (50, 60 and 80% from RD) on spinach yield and some content of nutrients as well as some parameters of soil fertility after harvest in a randomized complete block design. The addition of compost or bokashi plus 80 % of mineral fertilizer gave higher values of fresh and dry matter of spinach crop, as well as most nutrients content of the plant compared with raw rice straw. So, we recommend the addition of bokashi or compost with 80% chemical fertilizer to give the highest spinach yield and its constituents from some macro and micronutrients as well as in soil after harvesting under the same conditions.