This study aimed to determine the effects of alternation methods (AM) of drinking saline well water (SWW) with tap water (TW) on liver enzymes, blood minerals, and tri-iodothyronine in broiler chickens.
A total number 180 unsexed commercial broiler chicks Ross Alananny one day old were randomly divided into six equal groups, the first group, chicks drank TW containing 450 ppm total dissolved solids (TDS) from one day old to 36 day of age (the end of the experiment) and considered as control group (C).  Treatment 1 (Tr1), chicks drank SWW containing 3100 ppm TDS from one day old to the end of the experiment, Tr2, chicks drank SWW every other day alternatively with TW from one day old to the end of the experiment, Tr3, chicks drank SWW every other week alternatively with TW from one day old to the end of the experiment, Tr4, chicks drank TW during the first 18 days of age and drank SWW from 19-36 days of age, Tr5, chicks drank SWW during the first 18 days of age and drank TW from 19-36 days of age, Tr2 to Tr5 considered as alternation methods.
Total protein significantly decreased in all treatments when compared with C. Globulin was significantly decreased in Tr2, Tr4 and Tr5 when compared with C.
There were insignificant differences between AM treatments and C in alanine aminotransferase, Aspartate aminotransferase and triiodothyronine hormone. There were insignificant differences among Tr2, Tr3 and Tr5 when compared with C in sodium level.
In conclusion, drinking SWW containing 3100 ppm TDS for broiler chickens caused some negative effects on liver enzymes, blood minerals and tri-iodothyronine, Therefore, when it is necessary to use saline water, it is preferable to alternately drink SWW with TW every other week, or drink SWW during the first 18 days of age, followed by TW, to enhance these negative effects