The present study was conducted to investigate the effect of feeding different levels of Azolla and probiotics on growth performance and some blood biochemical of Indian River (IR) chickens. The study was conducted on 300 IR, which was randomly divided into ten treatment groups each with three replicates of 10 birds. The first treatment (T1) was fed a diet without Azolla while, Azolla 5% (T2), 10% (T3), 15% (T4), and 20% (T5), however birds in group 6 (T6) fed diet without Azolla and in drink water with 10⁸ Enterococcus faecalis, Azolla 5% with 10⁸ Ent (T7), 10% with 10⁸ Ent (T8), 15% with 10⁸ Ent (T9) and 20% with 10⁸ Ent (T10). The experiment lasted for 35 days then all animals were slaughtered. data indicated that body weight, body weight gain, feed intake and feed conversion ratio and liver functions (TP, ALT, AST, and ALP), lipids profile on cholesterol, triglycerides, and high density lipoprotein and low density lipoprotein, kidney function (uric acid and creatinine). The obtained results showed that,  a treated group with  T6 Entero ,(T2) 5%AZ, (T3) 10%AZ, (T7) 5%AZ+Ent and (T8) 10%AZ+Ent improve performance parameters with significantly increase in  BW, WG ,DWG and FI and significant decrease of FCR with the treated group with (T2) 5%AZ, (T3) 10%AZ, T6 Entero , (T7) 5%AZ+Ent and (T8) 10%AZ+Ent. As a result, the treated group with T2 (5% azolla), T3 (10% azolla), T6 ( Entero), T7 (5%azolla +Entero) and T8( 10% azolla +Entero) are the best treatment for growth  performance and biochemical parameter.