This study evaluated the sparing effect of betaine supplementation to low methionine laying hen diets on productive performance, egg quality, and nutrient digestibility.  A total of 250 Lohmann Brown laying hens, 25 weeks age were randomly distributed into 5 treatments of 50 hens in 5 replicates. Five tested diets were formulated T1: 0.45% methionine with no betaine supplementation which basal considered as a control diet, T2: 0.40% methionine with 0.75g betaine/kg, T3: 0.40% methionine with 1.5g/ betaine/kg, T4: 0.35% methionine with 0.75g betaine/kg, and T5: 0.35% methionine with 1.5g betaine/kg. Betaine supplementation to low methionine diets affected significantly (P<0.05) on FI, FBW and BWG. No significant differences (P>0.05) in EP, EW, EM, FCR, SW, AYW, HU, and EYC due to treatment effect. There was a significant difference (P<0.05) among groups on ST due to betaine supplementation to low methionine diet compared to no betaine control diet. Moreover, hens fed 0.35 and 0.40% methionine with betaine supplementation improved (P<0.05) immunoglobulin titre (Ig) compared to those fed on the control diet. Betaine supplementation to low methionine diets had significantly (P<0.05) affected on NB and all nutrients digestibility except CF digestibility compared to no betaine diet (T1). Methionine levels effect had effect (P<0.05) on FI, FCR, FBW, BWG, ST, EYC, and Ig titre. Decreasing methionine level up to 0.35% did not significantly (P<0.05) affected on all nutrients digestibility, except CP digestibility and NB which were affected significantly. Betaine levels effect significantly affected (P<0.05) on FI, HU, EYC, and Ig titre, while no effect (P>0.05) due to betaine levels on EP, EW, EM, FCR, FBW, BWG, SW, ST, and AYW. It could be concluded that betaine supplementation had a role-sparing effect with a low methionine diet especially at level 0.35% without any negative effect on laying productivity, egg quality, and immune response.