This research was done to find out the effect of in ovo injection of Coenzyme Q10 (CoQ10) at various incubation ages on the physiological and immunity performance of Mamora chickens. A total of 630 hatching eggs were divided into 7 equal treatments (90 eggs per each). The first group was as a negative control, the second group was as a positive control (injected in air sac with 0.3 ml/egg of distilled water at day 1 of incubation), the third and fourth groups were injected with 0.3 ml/egg of distilled water contained 0.1 and 0.2 ml coenzyme Q10 / egg respectively at day1 of incubation, the fifth group was as a second positive control (injected in air sac with 0.3 ml/egg of distilled water at day18), the six and seven groups were injected with 0.3 mL/egg solution of sterile distilled water contained 0.1 and 0.2 mL coenzyme Q10 / egg, respectively at  day18 of incubation, Hatched chicks of each treatment were reared till 28 days of age.      Results obtained could be summarized as follow: improving the hatchability percentage and decreasing embryonic mortality by in ovo CoQ10 at the 1st and the 18th day of incubation period as compared with negative control group. In ovo injection with CoQ10 led to improve the subsequent growth traits after hatch within the period of 1-28 day of age.  Lymphocytes (L, %) was elevated, while heterophils (H, %) was decreased for chicks hatched from eggs injected with 0.2 ml CoQ10 / egg at the 1st day of incubation. All chicks produced from injected eggs with CoQ10 had higher serum catalase enzyme value and lower liver enzymes (AST & ALT) compared with those of negative or positive control. Therefore, in ovo CoQ10 (0.1 or 0.2 ml/egg) injection improve hatchability, post-hatch chick development, and physiological response of hatched Mamora.