It is urgent to develop an ideal biopolymer with unique physicochemical and mechanical features that match its uses in different applications such as food packaging, wound dressing, and fuel cell membranes. Bacterial cellulose (BC) is a promissory biopolymer owing to its fascinating physicochemical features such as purity, biodegradability, biocompatibility, nanofiber structure, high crystallinity, water holding capacity and mechanical stability. One of the problems that limited the industrial production of BC is its low yield. A high-producing strain of this highly physicochemical biopolymer features is needed to benefit from its enormous uses in the food and medical applications. Bacterial cellulose (BC) synthesizing Komagataeibacter hansenii S strain was isolated from strawberry, and the 16S rDNA sequence homologies reached 99% with Komagataeibacter hansenii strain LMG 1527 so that the isolate can be identified as Komagataeibacter hansenii S. The isolated strain was selected owing to its high production yield of (5.4±0.6 g/l, dry weight) under optimized culturing conditions. The morphological and physicochemical features of the produced BC were studied by SEM, FTIR, XRD, TG, and mechanical tests. FESEM showed the three-dimensional porous nanofiber structure. FTIR and XRD confirmed the structure of BC. TG revealed the thermal stability of produced BC with good mechanical properties. Amoxicillin/ Flucloxacillin impregnated BC nanofibers exhibited high antibacterial potency against both Gram-positive and Gram-negative bacteria. Physicochemical and mechanical features of BC produced by Komagataeibacter hansenii S indicate its availability to be explored in food packaging, wound dressing, and fuel cell membranes.