This study was designed to investigate the effect of lemongrass powder and Orlistat on hyperlipidemia in rats fed on a high fat diet. Twenty four normal male albino rats of Sprague Dawley Strain weighing (150±10g), were used in this study and split into 4 equal groups; each group contained 6 rats.One group was conserved as a control negative group (G1), while (G2) were fed on high fat diet, (G3)were fed on high fat diet plus7.5 % lemongrass powder and (G4) fed on high fat diet plus Orlistat (60 mg /Kg body weight). At the end of the experiment, glucose levels, total protein (Albumin and globulin), serum liver functions, and serum lipids were assessed. The results indicated that tests plants improved glucose levels, liver functions, and lipid profile. According to these results, moderate amounts of lemongrass in our diets could be tried for improvement of hyperlipidemic humans.