Background: In the period of the COVID-19 pandemic, telemedicine has been a crucial instrument for the delivery of healthcare services. Its benefits include helping patients save time and money and protecting healthcare professionals from infection. Objectives: This study aimed to assess the knowledge and attitude of medical practitioners in Sohag governorate regarding telemedicine and related ethical issues after the COVID-19 pandemic. Methodology: A cross-sectional study was conducted, and data were gathered through a self-administered online questionnaire. The study was conducted in Sohag governorate over three months (from April to June 2022). Three hundred and twelve physicians of different specialties participated in this study. Results: About two-thirds of the participants knew the term telemedicine. Most participants believed that telemedicine could save transportation expenses and physician time. 59% of respondents thought that telemedicine could promote communication. About half of the participants believed that telemedicine threatened the privacy of patient information. Two-thirds of respondents said they should have patients' consent in written form. COVID-19 was an excellent chance to test telemedicine. According to most participants, the technical infrastructure is the main obstacle to a telemedicine application in Sohag governorate. Male physicians had better knowledge than females. Physicians aged 30-39 and consultants had a better attitude towards telemedicine. Conclusions: Physicians included in the study showed good knowledge and a positive attitude toward telemedicine. Following a brief trial period, doctors were ready to incorporate it into their practices. This study recommends that telemedicine conferences and training sessions be held, and telemedicine practice legislation and regulations be developed.