ABSTRACT
 
Background: The time that elapses after death of an individual can be estimated by observing certain events and changes that occur in the cadaver following the cessation of life. One such change involves the biochemical alterations in the components of the vitreous humor and synovial fluid. These changes can be identified by performing chemical analysis of these fluids. Due to the distinct separation of these fluids within their respective cavities, they are suitable for chemical analysis without being influenced by external factors or hazards. Aim: This research aims to estimate the postmortem period through measuring potassium and glucose levels in synovial fluid and vitreous humor.  Material and method: This work utilizes chemical analysis to detect and study the biochemical changes that occur in these fluids at 8, 16 and 24 hour postmortem intervals. By examining the levels of potassium and glucose in each cavity, researchers can estimate the postmortem interval, which refers to the time that has elapsed since the person's death. Results: The current study involved the examination of 53 deceased individuals who had been subjected to car accidents. Samples were collected from both the vitreous and knee cavities at 8, 16 and 24 h intervals following the accidents. These samples were then subjected to analysis to determine the levels of potassium and glucose. According to the findings of the study, there was a statistically significant and consistent increase in the levels of potassium, along with a progressive decrease in the level of glucose observed in both the vitreous humor and synovial fluid, which was directly proportional to the increase in time intervals. Conclusion: potassium and glucose analysis of aspirated vitreous humor and synovial fluid was a valuable tool for estimating the postmortem period.