Introduction: Suicidal attempts with hair dye containing paraphenylenediamine (PPD) have steadily increased over the last few decades. It contains PPD (highly toxic component) and a mixture of other chemicals that can damage the respiratory, muscular, blood, renal and hepatic systems. Objectives: This study aimed to analyze the laboratory findings following hair dye intoxication in Aswan University hospital. Methods: The current study was conducted in the internal medicine care unit of Aswan University Hospital involving fifty patients with acute hair dye poisoning in the period from September 2019 to March 2020 then continued from September 2020 till July 2021 due to Covid- 19. Some laboratory investigations as alanine transaminase (ALT), aspartate transaminase (AST), Urea, Creatinine and complete blood count (CBC) were recorded. Results: Liver function tests showed significant elevation of serum levels of ALT and AST. Renal function tests showed increase in serum urea and creatinine on the third day onwards. CBC changes occurred in form of increased total leucocytic count (TLC) while the hemoglobin, red blood cells (RBCs) and platelets count were normal. Conclusions: PPD-containing hair dye has emerged as a potential suicidal poison. It demonstrated a strong influence of laboratory markers indicating injury to vital organs of the body system.