Objectives : To investigate relation between antral follicle count (AFC) and chronological age to determine normal values for AFC in women with regular menstrual cycles.     
Study design : A Cross-sectional study was conducted on 575 women aged from 18–48 years with regular menstrual bleeding at EL-Shatby Maternity University Hospital to assess the AFC, scanning was performed in the early follicular phase. the results were compared with previous nomogram.   
Results: 575 normal menstruating females were categorized into 5 groups according to their ages and TVUS was done in the early follicular phase to detect the AFC. There's significant linear decline in mean AFC along different age groups in normal menstruating women with significant decline after age 38 years, There's a negative linear relationship between age and AFC in all studied centiles in normal menstruating women.     
Conclusion: the first step in providing patients with scientifically based counselling is the creation of a nomogram of AFC values for assisted reproductive technologies and also for the natural conception chance and pregnancy outcome.