Background: The growing global danger of antimicrobial resistance (AMR) to human health highlights the urgent need to develop new treatments for infections brought on by multidrug-resistant (MDR) bacteria. The dual impact of nanoparticles (NPs) is due to their ability to act as both an antibacterial agent and an antibiotic carrier is one of its advantages. Objectives: The goal of this work was to enhance the nanoparticle dispersing conditions by using different solvents or applying sonication, we also tested different microbial growth conditions and to examine whether using shaking vs. static conditions is more crucial for activity of NPs. Methods: Water, DMSO and acetic acid were used as solvents for testing NPs with or without sonication. The antibacterial activity of tested NPs was examined by twofold serial microdilution technique under shaking and static conditions. Conclusion: all the investigated NPs require sonication to generate a stable suspension; however, DMSO was needed for silver hydroxyapatite NPs and magnesium oxide NPs, water was needed for Zinc oxide NPs, and an acidic aqueous solvent, like 1% acetic acid, was needed for chitosan hydroxyapatite NPs. Meanwhile there was no difference between shaking and static conditions used in investigating the minimal inhibitory concentration of NPs.