Background: Genus Syzygium of the myrtle family comprises several aromatic species that are used for their aroma and edible fruits. Moreover, several Syzygium species exhibit antidiabetic, antifungal, anti-inflammatory, antibacterial, antioxidant, cytotoxic, antidiarrheal, anthelmintic, antinociceptive, analgesic and antivirus activities. Objectives and methods: The current study aimed to carry out the phytochemical screening for S. malaccense and S. samarangense leaves as well as isolation of the major phenolic compounds from 80 % aqueous methanol extract (AME) of S. samarangense using column chromatography and the compounds structures' were established by using chemical and physical methods. In addition, an investigation of the analgesic activity of the AME of two species was carried out for the first time using models of acetic acid-induced writhing test, hot plate test, and formalin-induced paw licking test in mice. Results: six phenolic compounds were purified from AME of S. samarangense viz methyl gallate (1), gallic acid (2), quercetin-3-O-β-D-glucopyranoside or quercetin-3-O-β-D-galactopyranoside (3), quercetrin (4), ellagic acid (5) andquercetin (6). Conclusion: two flavonoids, three phenolic acids, and one aglycone were isolated from S. samarangense leaves AME. Bothextracts are safe for the experimental animals, and they showed significant antinociceptive and analgesic activities peripherally and centrally in a dose-dependent effect. Therefore, the two species can be used for their analgesic activity.