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Phytochemical Study and Evaluation of the Antioxidant Activity of Aqueous and Ethanolic Extracts of <i>Hua gabonii</i>

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Last updated: 03 Jan 2025

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Section A: Natural Products & Metabolomics

Abstract

Objective: The aim of this study was to identify major phytoconstituents and evaluate the antioxidant activity of extracts from different organs of Hua gabonii. Methods: Phytochemical screening was carried out in order to highlight the major groups of secondary metabolites contained in the various extracts through TLC. To assess the antioxidant activity, three methods were used namely DPPH, ABTS and FRAP. The IC50 values for the various samples were determined using GraphPad Prism 9.0 software, while ANOVA and LSD multiple comparison test, were carried out using Statistix 10 software. Results: The findings showed that the ethanolic extracts of stem bark exhibited a higher activity with DPPH (IC50: 0.84±0.03 µg/mL) compared to the standards (0.81±0.15 µg/mL, 0.42±0.10 µg/mL and 0.33±0.05 µg/mL for gallic acid, catchin and quercetin respectively). In addition, the analysis by the ABTS test showed that leaf extracts also showed interesting activity (IC50: 13.86±0.29 µg/mL and 12.76 ±0.42 µg/mL for aqueous and ethanolic extracts respectively). The FRAP test revealed interesting activity for the aqueous extracts of leaves, stem bark and branch bark with IC50s of 0.006±0.00 µg/mL; 0.02±0.00 µg/mL and 0.02±0.01 µg/mL respectively, compared with 2,41±0.00 µg/mL for ascorbic acid. In addition, the TLC showed the presence of total polyphenols, phenolic acids, coumarins, flavonoids, anthocyanins, leuco-anthocyanins, tannins, quinones, alkaloids and terpenoids. Conclusion: While assay analyses revealed that the total polyphenol content was high in the ethanolic extracts and in particular in the stem extracts. In conclusion, H. gabonii can be considered as good sources of natural antioxidants.

DOI

10.21608/aprh.2024.225955.1230

Keywords

H. gabonii, extracts, Phytochemistry, Evaluation, antioxidant

Authors

First Name

Gédéon

Last Name

Bongo

MiddleName

Ngiala

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo. , Research Laboratory of Food and Nutrition (LARAN), Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa XI, Democratic Republic of the Congo.

Email

gedeonbongo@gmail.com

City

Kinshasa

Orcid

0000-0003-0710-0832

First Name

Clément

Last Name

Mbadiko

MiddleName

-

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo. , Research Laboratory of Food and Nutrition (LARAN), Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Koto-te-Nyiwa

Last Name

Ngbolua

MiddleName

-

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Sylvie

Last Name

Kasongo

MiddleName

-

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo. , Research Laboratory of Food and Nutrition (LARAN), Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Adrien

Last Name

Masunda

MiddleName

-

Affiliation

Institut Supérieur des Techniques Médicales/ISTM-Kinshasa, P.O. Box 35300, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Nadège

Last Name

Ngombe

MiddleName

-

Affiliation

Centre d'Etudes des Substances Naturelle d'Origine Végétale (CESNOV), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa XI, Democratic Republic of Congo.

Email

-

City

-

Orcid

-

First Name

Paulin

Last Name

Kapepula

MiddleName

-

Affiliation

Centre d'Etudes des Substances Naturelle d'Origine Végétale (CESNOV), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa XI, Democratic Republic of Congo.

Email

-

City

-

Orcid

-

First Name

Marie-Claire

Last Name

Yandju

MiddleName

-

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Pius

Last Name

Mpiana

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

First Name

Théophile

Last Name

Mbemba

MiddleName

-

Affiliation

Department of Biology, Faculty of Sciences, University of Kinshasa, P.O. Box 190, Kinshasa XI, Democratic Republic of the Congo. , Research Laboratory of Food and Nutrition (LARAN), Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa XI, Democratic Republic of the Congo.

Email

-

City

-

Orcid

-

Volume

8

Article Issue

1

Related Issue

45860

Issue Date

2024-01-01

Receive Date

2023-07-30

Publish Date

2024-01-01

Page Start

1

Page End

13

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 8_Issue 1_Pages 1-13.pdf

PDF . 612.7kB

Link

https://aprh.journals.ekb.eg/article_338710.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=338710

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1

Type

Research Article

Type Code

318

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

MainTitle

Phytochemical Study and Evaluation of the Antioxidant Activity of Aqueous and Ethanolic Extracts of <i>Hua gabonii</i>

Details

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Article

Created At

23 Dec 2024