Introduction: The objective of this study was to evaluate and compare the sealing ability of MTA, Nano-MTA and Biodentine in the repair of furcation perforation by using bacterial leakage test.


Methods and Materials: Standard access cavities and furcal perforations were made in fifty extracted mandibular permanent molars. The teeth were randomly divided into five groups. Group 1: perforations were repaired with MTA, Group 2: perforations were repaired with Nano-MTA and Group 3: perforations were repaired with Biodentine. Ten teeth with unrepaired perforations were used as positive controls and ten teeth without perforations were used as negative controls. The sealing ability was evaluated using Enterococcus faecalis Bacterial leakage test. The samples were observed for 45 days for turbidity using spectrophotometer.

Results: High statistically significant difference in leakage was found between the three materials when used as furcation perforation repair materials. Nano-MTA presented the best sealing ability with the least bacterial leakage followed by MTA, while Biodentine showed maximum bacterial leakage.

Conclusion: Nano- MTA has better sealing ability than MTA and Biodentine and can be used in repair of furcation perforation.