ABSTRACT
Aim:
This study was designed to evaluate the antimicrobial efficacy of Nano Silver Fluoride, Fluoridated Self-assembling peptide, and Sodium Fluoride Varnishes on oral Streptococcus mutans bacteria.
Materials and Methods:
The Antimicrobial efficacy was evaluated using agar-disk diffusion method. Three Muller-Hinton agar plates cultured with Streptococcus mutans (ATCC1402) were prepared. Each plate received three remineralizing agents (Group 1: Nano Silver Fluoride, Group 2: Fluoridated Self-assembling peptide;and Group 3: Sodium Fluoride varnish; as a positive control group) on three filter paper discs. Then the plates were incubated anaerobically for 24 h at 37°C. The diameter of the formed inhibition zones was measured in millimeters by a ruler to assess the antimicrobial effect. Statistical analysis was done using Kruskal–Wallis test and Mann–Whitney post hoc Test. The level of significance was set at P ≤ 0.05.
Results:
Both Fluoridated Self-assembling peptide and Nano Silver Fluoride had antimicrobial effects against Streptococcus mutans. However, the median value of inhibition zone size (mm) was greater in Fluoridated Self-assembling peptide 21 (18-22) as compared to Nano Silver Fluoride 9 (8-9), with a statistically significant difference (P value = 0.046). Enamelast Sodium Fluoride varnish showed no inhibition zones.
Conclusions:
Fluoridated Self-assembling peptide had a better antimicrobial effect compared to Nano Silver Fluoride and conventional Sodium Fluoride varnish against Streptococcus mutans.