Objective: the objective of the current study was to asses push out bond strength of Neosealer Flo in comparison to TotalFill BC and AH Plus sealers. Materials and methods: 30 recently extracted human mandibular single-rooted premolar teeth with only straight canals were included in the study. Samples were decoronated and root canals were enlarged using Protaper next up to X 4 then samples were allocated randomly into three experimental groups (n=10 per group) according to sealer type that was used in obturation; Group I: Neosealer Flo sealer, Group II: TotalFill BC sealer, Group III: AH Plus sealer. Samples were obturated using single cone technique with matched gutta percha cones. After obturation three dentin slices with thickness of 2-mm were horizontally cut at 2, 7 and 12 mm length from the coronal surface of each sample then push out test was performed on each slice by a universal testing machine. Results: AH Plus showed the statistically significantly highest push-out bond strength at coronal and middle third followed by Neosealer Flo while Total Fill BC showed the statistically significantly lowest push-out bond strength. Neosealer Flo showed the statistically significantly highest push-out bond strength at apical third followed by Total Fill BC and AH Plus where there was no difference between them. Conclusion: the highest push out bond strength at coronal and middle third was shown by AH Plus while at apical third the highest push out bond strength was shown by Neosealer Flo