Abstract
Aim: to quantify the amount of endogenous bone morphogenic protein 4 release under the effect of EDTA exposure for different time intervals.
Material and methods: Dentin scaffolds were randomly assigned to the following groups (n = 4). Group 1 received 17 % EDTA for 5 minutes; Group 2 received 17 % EDTA for 10 minutes; Group 3 received 17 % EDTA for 15 minutes and distilled water was used as a control in Group 4. After each treatment, ELISA was used to quantify the amount of BMP4 liberated from dentin scaffolds after pre-treatment with EDTA for 5, 10, and 15 minutes. The scaffolds were analyzed under SEM to investigate DPSCs attachment, morphology, the opening of dentinal tubules, and smear layer removal in the different groups.
Results: Dentin scaffolds conditioned with 17% EDTA for 10 and 15 min showed the maximum degrees of enlargement in dentinal tubules and smear layer removal, and revealed higher DPSCs attachment than the control group (p < 0.001), Moreover, dentin treated with 17% EDTA for 10 min manifested higher concentrations of BMP4 release than the unconditioned dentin (p < 0.05).
Using scanning electron microscopy, scaffolds treated with EDTA for 10 min presented the highest degrees of enlargement in dentinal tubules and smear layer removal than dentin treated with EDTA for 5 or 15 min.
Conclusion: endogenous bone morphogenic protein 4 release was the highest when dentin scaffolds were treated with 17% EDTA for a period of 10 minutes.
Key word: DPSCs, dentin scaffold, EDTA, BMP-4