Aim: The aim of this study was to compare the fracture resistance of endodontically treated teeth obturated with two root canal sealers using two different obturation techniques. Materials and methods: Forty freshly extracted single-rooted mandibular premolars were selected, decoronated, and mechanically prepared using EdgeFile X7 rotary files to size 40/0.04. Samples were randomly divided into 4 groups (n=10). In Group I: samples were obturated with AH Plus resin sealer using cold lateral compaction technique, Group II: samples were obturated with AH Plus resin sealer using single cone technique, Group III: samples were obturated with NeoSealer Flo bioceramic sealer using cold lateral compaction technique and Group IV: samples were obturated with NeoSealer Flo sealer with single cone technique.. Fracture resistance testing was done using a universal testing machine. The data were analysed statistically using one‑way analysis of variance followed by pair‑wise comparison using Tukey's post hoc test. Results: Neosealer Flo samples had significantly higher fracture resistance than AH Plus samples (p<0.001). Regardless of type of sealer used, single cone obturation technique had significantly higher fracture resistance than lateral compaction (p<0.001). Conclusion: Obturation using NeoSealer Flo sealer with single cone technique improved the fracture resistance of endodontically treated teeth.