Aim: The aim of this study was to assess and draw comparisons between the antibacterial properties of various bioceramic sealers.
Materials and methods: The samples were categorized according to the sealer type that was utilized. Group 1: Well Root ST, group 2: Ceraseal, group 3: Neosealer Flo, group 4: AH plus. Manufacturer's instructions were followed when preparing the sealers and placed in sterile plastic cylinders with a diameter and depth of 5 mm. Following that, specimens were incubated at 37°C and 100% humidity for 7 days. The bacterial suspension was made with a standardized density of 0.5 on the MacFarland (1.5x108/ml). The suspensions were diluted 10,000 times after mixing for six, fifteen, and sixty minutes, and then a triplicate amount of 0.01 from each diluted suspension was placed onto the pre-prepared BHI agar plates. (Difco Lab., Detroit, MI, USA). The number of colonies forming unit for each sealer was calculated in different time throughout the experiment. Results: For the effect on Enterococcus faecalis at 6 minutes, Neosealer Flo showed lowest bacterial count, at 15 minutes AH plus showed lowest bacterial count and 60 minutes Ceraseal showed lowest bacterial count. For the streptococcus mutans at 6 minutes, Neosealer flo showed lowest bacterial count, at 15 min AH plus and Well root ST showed lowest bacterial count and at 60 min well Root ST showed lowest bacterial count. Conclusion: All sealers showed antibacterial effect, where increasing the time resulted in decreasing Log10 CFU.