Abstract
Aim: The aim of this study was to demonstrate the amount of endogenous bone morphogenic protein2 released under the effect of EDTA exposure for different time intervals.
Material and methods: The dentin scaffold was randomly assigned to the groups (n=4). Group1 received 17 % EDTA for 5 minutes, while Group 2 received 17 % EDTA for 10 minutes. For 15 minutes, Group 3 received 17 % EDTA. Distilled water was used as a control in Group 4. After each treatment, a PBS wash step was performed, QuantikineTM ELISA was used to quantify the amount of BMP2 liberated from the dentin scaffold after pretreatment with EDTA for 5, 10, and 15 minutes، the scaffolds were analyzed under SEM to investigate the cell attachment, morphology, the opening of dentinal tubules, and smear layer removal.
Results:Dentin scaffold conditioned with 17% EDTA for 10and15 min showed the maximum degrees of enlargement in dentinal tubules and smear layer removal and revealed higher DPSCs viability than the control group (p< 0.001), Moreover, dentin treated with17% EDTA for 10 min manifested higher concentration of BMP2 release than the unconditioned dentin (p< 0.05).
Using scanning electron microscopy Dentin scaffold treated with EDTA for 10 min presented the highest degrees of enlargement in dentinal tubules and smear layer removal than the dentin treated with EDTA for 5or15 min.
Conclusion: Max endogenous bone morphogenic protein 2 release was obtained when dentin scaffold treated with 17% EDTA for a period of 10 min.
Key word: stem cell, dental scaffold, EDTA