Abstract
Introduction: The aim of the present study was to evaluate the antibacterial efficacy of two different powers of diode laser and photodynamic therapy PDT using Toluidine blue alone and in combination against Enterococcus Faecalis.
Methods: Seventy five extracted human maxillary central incisor teeth were chosen. Their crowns were removed at cementoenamel junction CEJ and the roots were mechanically prepared. After their sterilization, they were inoculated with Enterococcus faecalis for 72 hours. They were divided randomly into five equal groups according to type of treatment used. Group (I), high power diode laser with wave length 980 nm. Group (II), Toluidine blue TBO photosensitizer for five minutes. Group (III), low power 635 nm diode laser. Group (IV), Toluidine blue was activated by low power diode laser 635 nm. Group (V), Toluidine blue was activated by 980 nm diode laser.
Results: Antibacterial efficacy was assessed before and after treatment. 980 nm diode laser reduced bacteria by 86.41 %, Toluidine blue reduce it by 19.19%, 630 nm diode laser reduce it by 13.95%, toluidine blue plus low power 635 nm diode laser reduce it by 88.13 %, and toluidine blue plus high power 980 nm diode laser reduce it by 97.76 %.
Conclusion: The combined technique could be effective modality for elimination of Enterococcus faecalis from the root canals.