Aim: to evaluate and compare the cytotoxicity of NeoSEALER Flo, MTApex and AH Plus in both fresh and set states on human periodontal ligament fibroblasts over a period of one day, one week and one month.
Methods: The sealers were placed in standardized plastic rings to obtain identical sealer specimens. One group of specimens was tested immediately after mixing by preparing its extracts while the rest of the specimens were stored for 24hr at 37Cº. Human periodontal ligament fibroblasts were incubated with the extracts of the tested sealers. Cytotoxicity evaluation was done immediately after mixing, and for the extracts of one day, one week and one month according to immersion time. The cytotoxicity of all root canal sealers was determined using the MTT assay. Data were statistically analyzed using repeated measures ANOVA test followed by Bonferroni test and one-way ANOVA test supplemented with Tukey's test (P < 0.05).
Results: There was a statistically significant difference in cell viability presented by each tested sealer at different time intervals. Also, there were statistically significant differences in cell viability in all groups within the same time interval except for NeoSEALER Flo and MTApex at one month evaluation time. NeoSEALER Flo presented the highest cell viability percentages followed by MTApex and AH Plus respectively at each evaluation time interval.
Conclusions:
1- NeoSEALER Flo was the least cytotoxic while AH Plus was the most cytotoxic root canal sealer.
2- The increase in cell viability percentages was time-dependent.