Background: Virulence factors of denture stomatitis had been contributed mostly to Staphylococcus aureus and Candida Albicans as an etiological basis in addition to immuno-dysfunction related to type of denture base and significant Ab-Ag reaction according to individual immune response. This study aimed to evaluate gold electro plating effect on Staphylococcus aureus and Candida Albicans colonization in addition to assessment of its cytotoxicity level.

Subjects and Methods: Twelve maxillary completely edentulous patients were randomly divided into; Group I (control group): Completely edentulous patients rehabilitated with maxillary complete dentures reinforced with Cr-Co palatal plate and Group II (experimental group): Completely edentulous patients rehabilitated with maxillary complete dentures reinforced with Cr-Co palatal plate which were galvanic electro-plated by gold. Follow up assessment of Staphylococcus aureus, Candida Albicans and cytotoxicity level of Group I and Group II. The collected data were further statistically analyzed using SPSS and GraphPad statistical software.

Results: Using One Way ANOVA followed by Tukey`s post hoc test for multiple comparisons regarding microbiological evaluation, there was a significant difference among follow up periods for both groups. Using Independent t-test for comparison between both groups, there was a significant difference especially after two weeks, 30 days and 45 days. While for cytotoxicity level, there Gold-Plated experimental group showed insignificant higher cytotoxic effects than Cr-Co control group using Chi-Square test.

Conclusion: Gold electroplating showed an antibacterial effect and lower rate of Candida Albicans colonization while it showed higher levels of cytotoxicity.