Obtaining accurate linear measurements is of paramount importance in different dental fields, owing to their critical role in treatment planning and follow up of various procedures. The lack of accuracy of these measurements may lead to serious complications which might endanger the patient or adversely affect the treatment outcome. This leads the dental researchers to innovate new ways for ensuring the production of the highest possible precision levels in this domain. AIM: The aim of this study was to determine the accuracy of linear measurements obtained from 2-D MPR and 3-D VR of CBCT in comparison to direct skull measurements. Materials and Methods: In the present study twelve dry human skulls were conducted. Ten linear measurements were obtained between certain identified landmarks on each skull using a digital caliper. The skulls were subjected to CBCT examination. The measurements were obtained from 2-D MPR and 3-D VR of CBCT images, then compared to the real skull measurements and statistically assessed for accuracy Results: In the present study from the ten linear measurements evaluated from CBCT only three measurements from2-D MPR, while four measurements from 3-D VR images showed significant difference in relation to the gold standard . Conclusions: Linear measurements obtained from 2-D MPR and 3-D VR CBCT are highly accurate and reliable and can be confidently used for different maxillofacial clinical applications especially in orthodontics. CBCT linear measurements obtained from 2-D MPR images are relatively more accurate than those obtained from 3-D VR images.