Background: The impact of psychological setting at workplaces on employee health is still controversial. Working environments at hospitals may be extremely demanding and stressful. The aim of this study was to assess the work-related mental well-being of medical staff members. Methods: This is a cross-sectional study conducted at Cairo university's teaching hospitals. A convenient sample of 222 medical staff members at Kasr al-Ainy Medical School was included in the study. They were asked to fill a self-administered structured questionnaire on a Google Form. Results: A total 222 medical staff members participated, 73% were females and the majority were between 35 and 44 years. The majority (77.5%) were working in clinical departments while 22.5% were working in academic departments. The majority believed that their work environment didn't make them less confident about their achievement (60.8%) and were satisfied discussing novel concepts with their senior/manager (67.1%). On the other hand, 56.3% believed that workplace issues negatively affect their sleep and 29.7% spent their time searching for new positions. The majority (84% academic versus 77.9% clinical staff) suggested training on stress management and personal development as the most important item to relieve workplace stress. This was followed by mental health awareness campaigns (50% academic versus 17.4% clinical staff) and mental health insurance benefits and coverage (40% academic versus 24.4% clinical staff). Conclusion: Work environment culture affects peoples' mental health which reflects on both work productivity and job satisfaction. Stress relief strategies are important to improve staff mental health.