Background: Higher Education institutions in Egypt are currently performing several evaluations by both students and teaching staff as a measure to improve the quality of education provided. Thus, to retain the best and efficient work force, the Quality of Work Life (QWL) prevailing in these educational institutions needs to be investigated. So, this study was conducted to measure the level of QWL and its associated factors among teaching staff at faculty of medicine in Ain Shams University and to find out if there is any correlation between total QWL score and psychological distress including anxiety, depression, and perceived fatigue. Methods: Cross sectional study was conducted using convenient sampling technique to collect data from (210) faculty members from different academic and clinical departments. Three validated questionnaires including Total Quality OF Work Life (TQWL-42), hospital anxiety and depression scale and checklist individual strength questionnaire were used to collect data from participants. Results: The study indicated a satisfactory level of QWL among most of the study participants (84.8%), no significant difference in QWL among faculty members based on their personal and job characteristics. However, conducting multiple linear regression revealed that female gender and working in clinical departments were significant predictors for poorer QWL. Finally, the study found significant negative correlation between total quality work life score and anxiety, depression, and perceived fatigue. Conclusions: Most of the staff members enjoyed a satisfactory quality of work life. Being a female and working in clinical departments were the most important predictors for lower QWL.