Crayfishes, like other decapod crustaceans, possess certain biological traits that render them potentially valuable for aquaculture. In this field, stocking density, a key factor influencing crustacean aquaculture, is generally correlated positively with yields. This study aims at determining the optimal stocking density for mature freshwater crayfish, Procambarus clarkii collected from River Nile tributaries during March 2023. The specimens were transported and reared in the Marine Invertebrate's Laboratory at the Zoology Department, Faculty of Science, Al-Azhar University. A total of 520 individuals were maintained at three stocking density levels within an aerated circulation system. The findings revealed that a stocking density of 40 individuals/m2 was optimal for achieving rapid growth, ranging from 12.5% to 15.9% in total weight gain (TWG), with relatively low mortality (0-20%). Furthermore, increasing the stocking density to 80 individuals /m² showed promise yield, with a survival rate ranging from 80% to 92.5%. Notably, acceptable survival and growth rates may be achieved, depending on feeding mode and photoperiod. Additionally, the specific growth rate in weight (SGRW) was higher at a stocking density of 40 individuals/m² compared to 80 individuals/m². However, crayfish, like other freshwater crustaceans, is influenced by other factors that interact with stocking density such as molting, shelter cover, photoperiod, and aquaculture technique. Therefore, it is recommended to conduct further experiments for achievement more investments on this species.