The aim of this work is to isolate strains of Salmonella spp from broiler chickens and layer hens to identify their serotypes and to study their resistance profile.A total of 270 samples including liver and spleen were collected from 26 broiler farms and 25 layer hen farms. These samples include 140 broiler samples and 130 layer hen samples collected from different poultry farms in central Algeria. Strains of Salmonella spp were isolated and identified according to their macroscopic, microscopic and biochemical characteristics. The identification of the isolated strains was followed by serotyping of the isolates. Using Mueller-Hinton agar and the disk diffusion method, the resistance of the isolates to twelve antibiotic compounds was investigated. Among the 270 samples, 80 were positive (29.63%). In broiler chickens, 34 (24.28%) samples were confirmed positive, while in layer hens 46 (35.38%) samples were confirmed positive. Two serotypes from the 80 strains of Salmonella were identified with a predominance of the Salmonella Gallinarum serotype with a prevalence of 100% in layer hens and 79% in broiler chickens, followed by the Salmonella Enteritidis serotype. The highest resistances were recorded for Nalidixic acid NA (79.41%/100%), enrofloxacin ENR (41.18%/73.91%) and tetracycline Tcy (8.82%/54.35%), respectively in broiler chickens and the layer hen. However, no resistance to colistin was recorded in either species.48 isolates (60%) were multi drug resistant (MDR), and 3 antibiotic resistance profiles were recorded, with a multi-antibiotic resistance index (MARI) of 0.2 to 0.6.These findings demonstrated the risk that chicken farming poses to the persistence and spread of salmonella strains that are resistant to several antibiotics.