This study aimed to determine whether opuntia ficus indica and atriplex halimus-based diets can serve as potential fodder for Barbarine sheep in arid and semi-arid regions. it investigated the effect of using opuntia ficus indica and atriplex halimus l. as alternative diets on blood metabolites of Barbarine pregnant ewes. thirty-six barbarine ewes in their second half of pregnancy were randomly distributed into nine groups the control group included: 0.45 kg barley and 1.8 kg barley straw (d1). ewes in the experimental groups; d2 to d6, were fed barley straw ad libitum with 100% cactus, (75% c+ 25% atriplex), (50% c + 50% atriplex), (25% c + 75% atriplex), 100% atriplex. ewes on d7, d8 and d9 were fed 100% barley straw, 100% atriplex, and 100% cactus respectively. the results revealed a significant decrease in glucose in d8 and a significant decrease in cholesterol in groups d7 and d9 but increased significantly in group 8. triglyceride decreased in d2, d3, d4, d5, d7 and d9 and increased in d8. total protein and albumin decreased slightly in groups: d1, d3, d4 and d5. blood urea and creatinine decreased significantly in groups d7 and d9. the enzymes aspartate transferase and alanine transferase showed a significant decrease in d7 and d9 and a significant increase in d8 after feeding treatment. the results also showed a significant decrease in calcium and phosphorus levels in groups: d7, d8 and d9. it could be concluded that the mixture of opuntia ficus indica and atriplex halimus can be used as alternative diets for Barbarine pregnant ewes with no adverse effects.