This investigation has an evaluation objective of potential protective effects of α-Tocopherol (a form of vitamin E) and zinc sulfate against alterations in the levels of Cytochrome C and Caspase-3, which are crucial biomolecules involved in apoptosis (programmed cell death), within the brain tissue of Rats with albinism mutations (Rattus norvegicus) subjected to lead acetate (Pb(C2H3O2)2) exposure. This type of research is experimental research with a posttest-only control group design. The samples used were 25 female Wistar white rats with body weight of 250-300 grams and 4 months old which were divided into 5 treatment groups. The control group was given distilled water and corn oil, the P0 group was given corn oil, distilled water, and lead acetate at a dose of 1.5 mg/head, the P1 group was given α Add in 0,5 ml corn oil and Tocopherol at a dose of 100 mg/head distilled water 0.5 ml. The P2 group was given zinc sulfate 0.54 mg/KgBW add in distilled water and, corn oil 0.5 ml, and lead acetate 1.5 mg/KgBW, the P3 group was given zinc sulfate 0.54 mg/KgBW, 100 mg/KgBW α-Tocopherol, and 1.5 mg/KgBW lead acetate orally for 21 days. Based on the results of research and data analysis, it can be concluded that the combination of zinc sulfate and α-tocopherol can reduce the expression of Cytochrome C and Caspase 3.