Furano-coumarin compounds known as aflatoxins (AFs) are thought to be the most concentrated group of mycotoxin compounds, containing aflatoxin B1(AFB). Animal Feeds contaminated with AFB have a high risk for animal and human cancer. Therefore, it is essential to locate a microorganism or enzyme with extensive activity against the majority of mycotoxins. The study aims to impact the effect of LAB on broth medium by evaluating the potential efficacy of various spp of LAB for reducing AFB1. On the other hand, the safety tests and stages of LAB during the growth were done. Also, to evaluate the reducing ability of AFB1by LAB at 37°C and cold storage on in MRS. Lactic acid bacteria (LAB) have been broadly utilized in reduce aflatoxins in media it can reduce aflatoxins. The ability of five LAB in AFB reduction from broth MRS media greatly affected by several factors such as time of incubation, pH, temperature and inoculum size. 40°C was shown to be the ideal temperature for Lactobacillus plantarum to degrade AFB. Low deterioration was also attained at 4°C. The maximum reduction (58%) of AFB by Lactobacillus plantarum was obtained after incubation for 48 h. with inoculum size 10⁹ and pH 5 at 40 ⁰C. Also, reduction of AFB recorded 47 % and 46% for Lactobacillus rhamnosus and Lactobacillus bulgaricus after 48 and 72h. while, 30 and 25% of AFB reduction by the culture supernatant of Lactobacillus salivarius and Lactobacillus acidophilus at 40 ⁰C and pH 5 and after 72h.