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386544

Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

This study was conducted to investigate the potential therapeutic effect of S-methylcysteine (SMC) used singly, in combination with pyrimethamine (P) and sulfadiazine (S) regimen, and in conjugation with metal-organic framework nanoparticles (MOFs) on chronic toxoplasmosis in immunosuppressed mice. Fifty-four mice were immunosuppressed and divided into 9 groups (G); GІ (negative control), GП (positive control), GⅢ- GⅣf (infected and treated); GⅢ (P-S), GⅣa (SMC50), GⅣb (SMC25), GⅣc (SMC25, P-S), GⅣd (SMC25-MOFs), GⅣe (SMC25, P-MOFs, and S-MOFs), and GⅣf (SMC25-MOFs, P-MOFs, and S-MOFs). The infected mice were orally inoculated with 20 Toxoplasma cysts/ mouse. Mice treatment stared Fifty days post infection for 10 consecutive days. Assessment of the therapeutic effect of each treatment model included: parasitological parameters (brain cyst burden), histopathological examination of mice brain sections, and immunological assessment of mice serum interferon gamma (IFN-Υ) levels. GⅣf exhibited the highest percentage of brain cyst reduction (70.54%) and the highest percentage of IFN-Υ levels increase (182.23%). Nevertheless, significant increase in glial cell number, an evident increase in the degree of inflammation. Drugs loaded on MOFs have anti-Toxoplasma effect on chronic toxoplasmosis in immunosuppressed mice.

DOI

10.21608/ejvs.2024.313962.2327

Keywords

T. gondii, Pyrimethamine, Sulfadiazine, SMC, MOFs

Authors

First Name

Shaimaa

Last Name

El-Sayed

MiddleName

helmy

Affiliation

Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.

Email

shaimaa.helmy@med.helwan.edu.eg

City

-

Orcid

0000-0002-9858-2277

First Name

Ahmed

Last Name

Eassa

MiddleName

-

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

Email

ahmegy@hotmail.com

City

-

Orcid

0000-0002-1675-0231

First Name

Mousa

Last Name

Ismail

MiddleName

-

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

Email

dr.mousa.abdelgawad@gmail.com

City

-

Orcid

0000-0002-4514-2091

First Name

Marwa

Last Name

Elmallawany

MiddleName

-

Affiliation

Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

Email

mamallawany@hotmail.com

City

-

Orcid

0000-0002-1179-0162

First Name

Sara

Last Name

Abd Elhady

MiddleName

-

Affiliation

Medical parasitology department, faculty of medicine, Helwan university

Email

sara.mohamed@med.helwan.edu.eg

City

Cairo

Orcid

0000-0001-8190-5909

First Name

Reda

Last Name

Abdelhameed

MiddleName

-

Affiliation

Applied Organic Chemistry Department, National Research Centre, Giza, Egypt.

Email

reda-nrc@yahoo.com

City

-

Orcid

0000-0003-1700-5361

First Name

Ashraf

Last Name

Barakat

MiddleName

Mohamed

Affiliation

Department of Zoonotic Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth str. Dokki, Giza, Egypt

Email

ashrafbarakat2@gmail.com

City

Giza

Orcid

0000-0002-3576-5738

Related Issue

-2

Receive Date

2024-08-21

Publish Date

2024-10-16

Page Start

1

Page End

12

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS3865441729026000.pdf

PDF . 1.3MB

Link

https://ejvs.journals.ekb.eg/article_386544.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=386544

Order

386,544

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice

Details

Type

Article

Created At

23 Dec 2024