This study explored the biosynthesis of silver nanoparticles (AgNPs) using Urginea maritima follicles extract (UMBE) as a reducing agent, to develop a therapeutic application. Furthermore, the current study represents the clinical application of the UMBE and its prepared nanoparticles and the impact of both factors on the levels of various biomarker parameters (metabolic, antioxidant and oxidant) in the sera of female albino rats with induced diabetes was evaluated. The outcomes indicated that the levels of metabolic biomarkers (glucose and total lipid) were significantly increased in diabetic groups while the total protein level decreased compared to the healthy group. Whereas the antioxidant markers, have shown significantly drop in their sera concentration in diabetic rats compared to the healthy group. Significantly, both UMBSNPs and UMBE demonstrated the ability to enhance the activity of antioxidant enzymes in the diabetic group when compared to the healthy group. Similarly, the oxidant markers increased in diabetic rats compare to healthy ones with a significant change. Based on the latest findings, it is evident that UMBE and UMBSNPs have the potential to enhance antioxidant status and mitigate the oxidative impact associated with diabetes mellitus, suggesting their promise as an alternative treatment.