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376221

Genetic Diversity of GPAM, DGAT1, and SCD1 Lipogenic Candidate Genes In Egyptian and Indian Buffalo

Article

Last updated: 01 Jan 2025

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-

Tags

Animal Reproduction & Artificial Insemination

Abstract

The objectives of the present study were to analyze polymorphisms in the glycerol-3-phosphate acyltransferase mitochondrial (GPAM), diacylglycerol O-acyltransferase 1 (DGAT1), and stearoyl-coenzyme A desaturase 1 (SCD1) genes in the Egyptian buffalo breed, and also to explore genetic diversity across Egyptian, Murrah, and Bhadawari buffalo populations. Genotyping was conducted using PCR-SSCP and sequencing techniques. Results indicate significant deviations from Hardy–Weinberg equilibrium (HWE) in the Egyptian buffalo breed for GPAM, DGAT1, and SCD1 genes, with Murrah significantly differing at DGAT1 and SCD1 genes. Bhadawari breed significantly deviated from HWE only at DGAT1 gene. Pairwise comparison between populations revealed the shortest genetic distance between Egyptian and Murrah, while the longest between Bhadawari and Murrah. Multiple SNPs were identified in the genes under study, with alterations of amino acid sequences. These findings reveal novel genetic variants with potential implications for lipid metabolism and milk quality in Egyptian buffalo. Further research is warranted to elucidate the functional significance and breeding applications of these variations.

DOI

10.21608/ejvs.2024.302279.2234

Keywords

River buffalo, GPAM, DGAT1, SCD1, PCR-SSCP, SNPs, Genotyping

Authors

First Name

Sonia

Last Name

Sihag

MiddleName

-

Affiliation

Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana, India.

Email

soniasihag7194@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Zahran

MiddleName

A

Affiliation

Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt.

Email

mohamed.zahran@agr.cu.edu.eg

City

Giza

Orcid

0009-0003-8108-6810

First Name

Hamdy

Last Name

Abdel-Shafy

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt.

Email

hamdyabdelshafy@agr.cu.edu.eg

City

-

Orcid

-

First Name

Vinod

Last Name

Chhokar

MiddleName

-

Affiliation

Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana, India.

Email

vinodchhokar@yahoo.com

City

-

Orcid

-

First Name

Anil

Last Name

Kumar

MiddleName

-

Affiliation

Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana, India.

Email

bhankhar@gmail.com

City

-

Orcid

-

First Name

samy

Last Name

Abou-Bakr

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt.

Email

samyabm2006@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Hassanane

MiddleName

s

Affiliation

Cell Biology Department, National Research Center, 12622 Dokki, Giza, Egypt

Email

hmsaber@yahoo.com

City

-

Orcid

-

First Name

Hossam

Last Name

E. Rushdi

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt.

Email

hosamrushdi@agr.cu.edu.eg

City

GIZA

Orcid

-

Related Issue

-2

Receive Date

2024-07-07

Publish Date

2024-08-26

Page Start

1

Page End

14

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS3762211724619600.pdf

PDF . 1.7MB

Link

https://ejvs.journals.ekb.eg/article_376221.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=376221

Order

376,221

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Genetic Diversity of GPAM, DGAT1, and SCD1 Lipogenic Candidate Genes In Egyptian and Indian Buffalo

Details

Type

Article

Created At

23 Dec 2024