The aim of the research is to study the mechanism of neurotoxic behavioural changes of scopolamine in the mice. Number of 15 animals, divided into 3 groups, each group consisting of 5 animals. The first group was considered a control group. The second and third groups were given scopolamine in two doses of 10 and 20 mg/kg and were injected once, and then behavioural measurements were taken 24 hours after treatment. Impact of acute high single doses treatment with scopolamine in behavioural tests for higher brain functions there is a significant increase in rearing and decrease activity in crossing numbers in the 10, 20  mg/kg.  Both doses significantly affect the Social Interaction behaviour of mice compared to the control group after 24 hours of acute single doses.  Both doses of scopolamine significantly effect on swimming scour and tail suspension, both the 10 and 20 mg/kg exhibited significant increases in serotonin levels compared to the control group. Additionally, the 20 mg/kg group showed a significance compared to the control and 10 mg/kg group.  Both the 10 and 20 mg/kg exhibited significant decreases in acetylcholine levels compared to the control group.   20 mg/kg exhibited a significant decrease in COMT levels compared to the control group. The scopolamine has an effect on the neurobehavioral of animals. The results showed that these effects have a direct relationship to neurotransmitters, including acetylcholine and serotonin, as well as the effect on the COMT enzyme.