Fascioliasis  caused by Fasciola is commonly reported in cattle. Various studies have evaluated the use of somatic antigen for immunodiagnosis of fascioliasis, but the results on the performance remain not clear. So, the present study was conducted to evaluate the performance of IgG-Enzyme-linked Immunosorbent Assay (ELISA) using total somatic antigens of Fasciola to diagnose fascioliasis and the effect of regulatory cytokines in cattle. A total of 428 serum samples from Fasciola spp infected cattle following the postmortem examination (coprology and liver incision) were tested for the performance of IgG-ELISA. For cytokines analysis, 150 serum samples (87 seropositive and 63 seronegative) were examined. Out of 428 samples, only 87 were diagnosed positive using the IgG-ELISA, given a performance of 20.32%. Season, sex and age did not show significant influence on the seropositivity. However a high IgG level was observed in rainy season than dry season (p < 0.0009). Seronegative Fasciola infected cattle had highly elevated serum transforming growth factor-beta (TGF-β) (p<0.0001), low serum Interleukin (IL)-10 (IL-10) (p < 0.0001), but no difference in serum interleukin (IL)-4 (p = 0.62) level. Furthermore, there was significant (p<0.0001) high level ratio of TGF-β/IL-4 in seronegative. The ratio TGF-β/IL-4 was negatively correlated with IgG level in seropositive Fasciola spp. infected cattle. It was concluded that overall somatic antigen did not show a convincing diagnosis of fascioliasis and this low performance may be associated with changes in TGF-β and IL-10 levels.