This study was conducted to evaluate the effect of adding multi-strain probiotics (MSP) with or without dry yeast to the diet on blood, immune characteristics and antioxidant status of male Saidi sheep. Five males (54.14 ± 1.67 kg average body weight) were used and randomly distributed in a 5 × 5 Latin square design to evaluate MSP administration for a period of 105 days. The five treatments were given the basic diet (50:50% rough/concentrate), where the first treatment was control and without other additives, while 2^nd and 3^rd groups were added at a rate of 1 g/day/animal to the other four treatments at a level of (2 x 10⁹ cfu/g) or (4 × 10⁹ cfu/g), ABLB2 and ABLB4, respectively. The 4^th (ABLB2+SC) and 5^th (ABLB4+SC) treatments were ABLB2 and ABLB4 enriched with 1 g/day/animal of dry yeast Saccharomyces cerevisiae (SC) was added at a level of (2 × 10⁷ cfu/g). The results showed that adding MSP to the basal diet had no effect on blood parameters. While ABLB4 and ABLB4+SC groups had better values ​​of GSH-Px and TAC and significantly reduced the level of MDA compared to the control (P < 0.001). There was a slight improvement in the IgM concentration, along with a significant increase in IgA and IgG levels, as well as lysozyme activity in the blood serum in all MSP groups. In conclusion, adding multi-strain probiotics with yeast to the basic diet enhanced the antioxidant capacity and immune response in blood serum in Egyptian male sheep.