Applications for cancer treatment widely use reliable cyclosporine compounds combined with the organic polymer chitosan nanoparticles. The surface chemical characteristics of the chitosan/cyclosporine nanoparticles were investigated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and particle size (PS) measurements. Field-emission scanning microscopy (FESM) was used to obtain morphological images of the composite materials. The homogenous composition and interaction between chitosan and cyclosporine performed well for future studies, supporting this study are its nanoparticle applications. The proposed chitosan nanoparticle manufacturing process is simple, low-cost and environmentally friendly. Moreover, these advantages indicate the possibility of using interesting samples for cancer treatment applications in advanced biomedical and practical fields.