The poultry industry is the most important part of the human food supply because it is a rapid production process that supplies cheap sources of protein in food, so it is considered the most significant sector in the economy, especially in a growing country. This study aimed to investigate the changes that occur in the level of interleukin IL6, IL4, and interferon-γ after using flaxseed and Omega-3 oils in broilers vaccinated with Newcastle disease by using ELISA test as a method. The 240 one-day-old chicks were divided into eight groups and treated either with a Newcastle vaccine or by vaccine and 1% of flaxseed or/and omega 3 oils, blood was collected after 1,3,10,17,24, and 31 days post-vaccine. our results explain a significant increase in all immunological markers (interleukin IL6, IL4, and interferon-γ) especially in groups treated with oils and reaching higher concentration on the 17th and 24th day post vaccination. We conclude that both oils (flax seed oils and omega 3) have augmenter effects on interleukin response and can modulate broiler immunity to provide a good immune state after the vaccine with Newcastle which may give more protection against infection.