Crimean-Congo Hemorrhagic Fever (CCHF) nowadays one of challengeable zoonotic disease both for breeding and human societies. The disease caused by   CCHF virus. It is severe disease with high mortality rate to human. Our study was aimed to detect specific antibodies against CCHFV both in cows and their owners by ELISA technique in Nineveh province in Iraq.  A total of 124 sera were collected from cows in various ages, genders, and breeding methods that distributed within the Nineveh province including Al-Gyarah, Makhmoor, Al-Hammam, Al-Shora, Zammar, in addition to Hatra cities. In addition to obtaining the same number of samples (124) in different ages and genders from the owners of these cows. The study results showed that overall seroprevalene of CCHFV in cattle was 60+ /124 (48.4%). The prevalence of CCHF was highest in age group 5-7 years  26+/39 (66.6%)  compared with other age groups 1-2 years 6+/18 (33.333) and 3-4 years 28+/67 (41.791).A 56 cow sera out of 117 were found seropositive (47.9%) while 4 ox sera out of 7 were seropositive (57.1%) against CCHFV. Our study showed that the seroprevalence of CCHFV  in inbreeding system 35+/68(52.5%)was higher than that in outbreeding system cows 25+/56 (44.6 %).The overall seroprevalence in owners was 6+/124(4.8%)with sero positive rate of 3+/60 (5%) in male and 3+/64(4. 7 %)in female genders. It was concluded that CCHF was present in different areas of  Ninavah province Iraq country both in cows and their owners with different seroprovelance rate in both of them with different genders and sex.