The experiment was conducted for the period from (9/16/2023 to 11/18/2023), with the aim of studying the effect of injecting eggs with different levels of organic selenium and spirulina algae extract on the blood biochemistry of broiler chickens. The experiment of injection and hatching of chicks was conducted in the Lailani hatchery located in the Lailan area, which contains BROODTECH type hatcheries. The duration of the experiment was 21 days. (600) hybrid eggs (Ross 308) from a flock of 33-week-old hens imported from Turkey were used. The average egg weight was 58 g according to the certificate of origin. They were randomly distributed among (6) treatments with (100) eggs for each treatment and placed in BROODTECH incubator drawers until the age of (18 days), after which the eggs were injected and the drawers were transferred to the hatchery until the chicks emerged at the age of (21 days). The parameters used were as follows: T1 = no negative control injection T2 = injection of 0.5 ml of deionized distilled water per egg (positive control) T3 = injection of 0.5 ml/egg containing a concentration of 10 micrograms of organic selenium T4 = 0.5 ml/egg injection containing a concentration of 20 micrograms of organic selenium T5 = 0.5 ml/egg injection containing 1.5 mg of spirulina algae extract T6= 0.5 ml/egg injection containing 3 mg of spirulina algae extract. The study conducted that inject the egg with organic selenium and Spirulina were effected on the level of both calcium and phosphor, and decrease the MDA in the blood serum.