This study was designed to determine the type of bacteria in animal that my cause diarrhoea.  This study includes 100 samples   form sheep and cows, which were collected from private veterinary clinics. These samples were transferred to this laboratory, and all the bacterial isolates were diagnosed based on microscopic and phenotypic characteristics and biochemical tests. The E. coli bacteria was recorded the highest percentage of 59 [47%] of the bacterial isolates followed by Proteus spp. which recorded 36 [26%], while both bacterial isolates of Pseudomonas spp. and Salmonella enterica were 18 [13%] and 8 [5%], respectively. Out of 100 fecal samples from cultured calves and sheep, 126 bacterial isolates were examined and obtained. The E. coli bacteria recorded the highest percentage of 59 [47%] of the bacterial isolates.  E. coli. Was recorded the highest percentage in the samples from children and animals [calves and sheep], they were 56% and 47%. The diarrhea samples   contained different types of bacteria   where the 65 [65%] of the samples were contained E. coli and 15 [15%] of them were contained Salmonella enterica and E. coli. [47%] of the stool samples were detect strains of E. coli bacteria. All isolates [47%] contained the uidA gene which confirmed the identification of E. It was found that the most important type of bacteria that causes diarrhea in children and might be transmitted from animals was E. coli. The most important diarrhea-causing strains of E. coli isolates in sheep calves were ETEC and EHEC.