The protozoan parasite Entamoeba histolytica is the causative agent of amebic colitis, an infectious illness that kills over 100,000 people annually throughout the world.. this study aimed to diagnosis of amoebiasis using an smear direct microscope and PCR assay, assessment of some hormone markers includes. Leptin, obesatatin and IGFBP2 by ELISA. the research was conducted from July 2023 to November 2023.Atotal of 563 stool samples for infants and children those who suffered from diarrhea and abdominal pain in Ibn Al-Atheer Hospital, Al-Khansaa Hospital, and Al-Salam Hospital in governorate of Nineveh. Examined using an direct microscope 100 positive samples (18 %) and 463 negative samples (82 %) Detection of the DNA of amoeba by specific genes primers by PCR method. 74 positive samples were observed out of 464 examined. There was a considerable increase in Leptin levels. in patients along with an average concentration of 4.4±1.6 ng/mL, compared to the control group, whose average concentration 1.4±0.4ng/mL, obestatin levels in patients were signified greater with a mean of 188.1±85.04ng/mL, compared to the control group whose average concentration 49.2±11.5 ng/mL, and increase in IGFBP2 levels. in patients along with an average concentration of 49.8 22.4ng/mL, compared to the control group whose average concentration 14.4±5.3ng/mL.: The study revealed that patients had increased levels of leptin, obestatin, and IGFBP2 when the infection of E.histolytica occurred .