The study aimed to determining the prevalence of Cryptosporidium spp. among different groups of residents in Al-Anbar Province. The Fallujah Teaching Hospital, Al-Ramadi Maternity, Children's Hospital, IDP camps, prestigious medical facilities in Al-Anbar Province, and privately held laboratories were among the medical facilities from which these individuals sought care. Children, adults, boys, and girls were represented in the 420 stool and blood serum samples that were collected and sent to private laboratories. A 14.3% Cryptosporidium spp. infection was discovered in 60 of the individuals who had been examined. Based on microscopic, 360 individuals tested negative for Cryptosporidium spp. indicating an 85.7% non-infection proportion. The study comprised 28 (46.7%) men and 32 (53.3%) women. Notably, the greatest infection incidence was seen in the age category of 1 to 14 years, with 21 people (15.3%) afflicted. Regarding residence, 37 patients (15.7%) were from rural regions, whereas 23 (12.5%) were from metropolitan areas. The study also found that Cryptosporidium spp. infections are seasonal, with the largest infection rates (31.80%) occurring throughout the summer months of April 2022, July 2022, and August 2022. Furthermore, the study used ELISA testing, which found that 22 serum samples (22.9%) were positive. IgM ELISA findings showed a 10.40% positive rate, whereas IgG results showed a 12.50% positivity rate for Cryptosporidium spp. These data were acquired by the ELISA technique.